After replacing the check valve, prime the pump and perform the static leak test. The acquity uplc mclass system expands the performance of any nano to microscale ms analysis. Before changing modes from the manual tuning window, all instruments were reset from the instrument system status section, and the workstation was reset in device management. Demonstrates how to replace the check valve in an acquity uplc bsm. Using the acquity uplc columns calculator, it is then scaled to uplc and optimized for the shortest analysis time at equal peak capacity. Ultra highperformance liquid chromatography, uhplc, uses columns packed with porous sub2m particles usually 1. The hclass and acquity, differ in the dwell volume of the system, the hclass has the larger volume and thus the acquity uplc system has a faster injector cycle time and less of an equilibration time, it is better for ballistic short and steep gradients, but the run times have to. The scientific tools you deploy, and the information it provides, can make all the difference. The most powerful binary uplc system, with the best dispersion, on the market. Acquity uplc h class system lowdispersion system delivers true uplc performance to improve chromatographic resolution, increase sensitivity, and help improve your sample throughput range of features and tools to give you the maximum versatility for method development. Millennium and waters are registered trademarks, and acquity uplc. Waters acquity uplc iclass with fd and pda detectors.
Waters acquity uplc hclass system conquer scientific. Acquity uplc i class sample manager flow through needle smftn cause drawing sample rate excessive can be caused by the syringe draw rate being too fast, possibly because the solution is viscous. See acquity uplc iclass systems specification guide for conditions. Acquity iclass thfhexane 7000049 acquity qsmr 700010664 for i2v systems, if replacing the check valve does not correct the problem, replace the i2v actuator. Offering higher sensitivity whilst increasing analytical speed, waters then introduced their h class in 2010, aimed at easing the transition from traditional hplc methods. Share, ask, and interact with peers from around the globe in dynamic discussions. The acquity uplc iclass plus system provides new possibilities of maximizing the resolution and sensitivity of your methods. Difference between acquity uplc and acquity uplc hclass.
The new acquity uplc iclass system takes separations technology to the next level by maximizing peak capacity and extending the performance of any ms detector. It increases the linear dynamic range of your analysis, resulting in better ms analyses by dda or ms e. This video describes how to replace the sample needle, needle guide, and vespel seat in the acquity hclass sample manager flow through needle. Waters acquity uplc hclass series system manual pdf download.
The acquity uplc i class plus system provides new possibilities of maximizing the resolution and sensitivity of your methods. Waters uplc i class system sm fl performance specifications. Xevo g2s system vacuum pump cable kit lockspray source with esi probe masslynx performance workstatio. Apr 10, 2018 introducing the new acquity uplc i class plus system. Waters acquity uplc iet refurbished analytical laboratory. Get high quality hplc replacement parts of waters, acquity at sciencix with cost effective rates.
The reversedphase qc reference material is designed for use with any chromatographic systems containing a uv detector. How to install a uplc column onto an acquity uplc system with an active column preheater. However, neither resets worked to resolve the switch to operate function. Waters designed the new acquity uplc hclass plus, hclass plus bio, and iclass plus systems for unparalleled flexibility, performance, and easeofuse. Waters asr catalog waters reversed phase qc reference. Intended use of the acquity uplc hclass system the waters acquity uplc hclass system is for research use only. Qualification of laboratory instrumentation is an integral part of compliance with regulatory and quality standards such as gmp, glp, iso 17025, usp, and others. The high pressure 18,000k psicapable acquity uplc i class plus system has been purposefully engineered for the lowest dispersion, and with an extended pressureflow envelope, complex separations can be accelerated without compromising chromatographic fidelity. Corporation assumes no column manager with active preheater. The waters acquity uplc system was used in the lab for both offline connected to a maldi spotter and online connected to an esi instrument separation of peptides. The maintenance steps most frequently used are shown in stepbystep videos on the waters homepage. Intended use of the acquity uplc hclass bio system the waters acquity uplc hclass bio sy stem is for research use only and is not intended for use in diagnostic applications.
Apr 10, 2018 the acquity uplc i class plus system is a binary uplc system designed to generate ultraefficient, narrow peaks and to improve the sensitivity of optical detection or mass spectrometry. Acquity uplc iclass system datasheet waters corporation. Calibrating to calibrate lc systems, follow acceptable calibration methods using at least five standards to generate a standard curve. When operating the acquity uplc instruments, follow standard qualitycontrol qc procedures and the guidelines presented in this section. New waters acquity iclass bsm performance maintenance kit 20260. Describes how to prime the seal wash and pump in an acquity uplc bsm. Acquity, acquity hclass bio qsm, acquity hclass qsm, acquity iclass bsm, acquity isocratic solvent mgr, acquity mclass asm, acquity mclass bsm, acquity uplc bsm, acquity uplc i2v bsm, nanoacquity, nanoacquity uplc bsmasm. Ultra high performance liquid chromatographs uhplc systems. Applicable symbols audience and purpose this guide is intended for personnel who install, operate, and maintain acquity uplc instruments. The hilic qc reference materialqcrm is designed for use with uv and ms chromatographic systems using a hilic column. Waters, acquity iclass bsm hplc replacement parts by.
It gives an overview of the systems technology and operation. The acquity uplc iclass plus system supports waters empowerbased automated system qualification tool sqt, which minimizes the time and cost associated with annual qualification. Acquity uplc iclass system smfl waters corporation. Intended use of the acquity uplc system waters designed the acquity. Attachments waters acquity uplc h class system instrument specifications 347 kb. How to replace the sample needle on an acquity flow. Acquity uplc hclass series laboratory equipment pdf manual download.
When used consistently and control charted, the qcrm will provide confidence in your results reduce troubleshooting and rerun time enable cross lab result comparisons it is adaptable to a wide variety. Acquity h class bio smftn, acquity i class bsm, acquity uplc. The acquity uplc i class system supports waters empowerbased automated system qualification tool sqt, which minimizes the time and cost associated with annual qualification. For the usp assay of clozapine, the system suitability solution was run on both an agilent 1100 lc system and the acquity uplc h class. The waters acquity uplc iclass systems holistic design is targeted for. Page 43 using the waters acquity uplc columns calculator ensures the best results for transferring the lc method from hplc to uplc or uplc to hplc. As a member of waters community forum, you can build your technical skills and professional network. This acquity classic uplc binary system from waters is in excellent condition, and comes with complete oq certification see photos, including fully reconditioned and serviced. An overview of the patrol uplc process analysis system, which gives process scientists the ability to make decisions in a timely fashion, throughout the entire product lifecycle. Acquity uplc iclass plus system from waters selectscience.
Looking for answers to the most complex scientific challenges is what you do. The directnon split automatic solvent flow control algorithms of the acquity uplc mclass system provide pulseless flow for increased retention time reproducibility. For the usp assay of clozapine, the system suitability solution was run on both an agilent 1100 lc system and the acquity uplc hclass. While its true that the acquity uplc and the acquity uplc h class have different system volumes, whats important to understand is that only the system components through which the sample passes contribute to band broadening resulting in loss. By embracing a philosophy of continuous product improvement, along with customer feedback, waters designed the new acquity uplc hclass plus, hclass plus bio, and i. Waters designed the acquity uplc system to isolate, concentrate, separate, detect.
The waters acquity tqd features the highest levels of tandem quadrupole ms selectivity, robustness, speed, and accuracy for quantitative analysis. Acquity, acquity cm30s, acquity hclass bio qsm, acquity hclass bio smftn, acquity hclass qsm, acquity iclass bsm, acquity uplc bsm, open architecture uplc, nanoacquity, nanoacquity hdx mgr, nanoacquity uplc bsmasm. Waters acquity uplc i class with fd and pda detectors. Get high quality hplc replacement parts of waters, acquity iclass bsm at sciencix with cost effective rates. Wat today introduced the new waters acquity uplc iclass system, the highest performing uplc system ever engineered taking ultraperformance lc separations technology to the next level. The high pressure 18,000k psicapable acquity uplc iclass plus system has been purposefully engineered for the lowest dispersion, and with an extended pressureflow envelope, complex separations can be accelerated without compromising chromatographic fidelity. Protection class i the insulating scheme used in the instrument to protect from electri.
Waters introduces highest performing uplc ever engineered. The acquity uplc hclass is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flowthroughneedle injector to deliver the advanced performance expected of uplc type separations high resolution, sensitivity and improved throughput while maintaining the robustness and reliability. Three new acquity ultraperformance lc uplc systems that build off of the success of waters industryleading acquity uplc plus separations platform. Acquity waters xevo g2s has recently come off lease and is available for shipment to your laboratory. Acquity, acquity hclass bio qsm, acquity hclass bio smftn, acquity iclass bsm, acquity uplc bsm, open architecture uplc, nanoacquity, nanoacquity uplc. Symbol definition manufacturer authorized representative of the. I was personally involved in this project and i am very excited to say that we have advanced this already extraordinary uplc technology even further.
Waters column manager manual 845 gelmek learn how to. Your research could lead to groundbreaking medicines, cleaner drinking water or. Prime the sample syringe for five cycles and ensure that liquid is coming out of the peek fitting you removed from port 3. Waters has turned what was a highly manual analytical process, where success was dependent on technical skill, into an integrated and automated system solution based on the nanoacquity uplc system with hdx technology. Arc has been running every day for almost two years, making a minimum of 40 injections of complex samples of hemp extr. Waters acquity uplc hclass system transform standard procedures into ultimate performance. Page 44 using the waters acquity uplc columns calculator ensures the best results for transferring the lc method from hplc to uplc or uplc to hplc.
Waters acquity uplc iclass plus system waters corporation. As usual, the equipment from waters did not disappoint me. The acquity uplc i class features decreased system dispersion for higherresolution separations and highest peak capacity. The qda qc reference materialqcrm is designed for use with waters acquity qda detector. Remove both port 4 and port 3, and port 2 fittings on the inject pod. No flow coming from acquity bsm qsm wkb46463 waters. When you develop methods, follow the protocol for the adoption of analytical. Acquity uplc iclass sample manager flow through needle smftn cause drawing sample rate excessive can be caused by the syringe draw rate being too fast, possibly because the solution is viscous.
This waters acquity uplc hclass system is in excellent condition the acquity uplc hclass system is the only quaternary based liquid chromatographic system that was designed for true uplc performance without compromise. Uplc performance is redefined with the acquity uplc iclass system, delivering the highest chromatographic resolution and sensitivity with the lowest lc carryover. The reversedphase qc reference material is significantly more complex than the neutrals qc reference material in that it contains a void marker, neutral, acidic and basic compounds which could be challenging to. In the console, select the sample managerftn from the system tree.
View and download waters acquity uplc hclass series system manual online. Jul 25, 2011 uplc performance is redefined with the acquity uplc i class system, delivering the highest chromatographic resolution and sensitivity with the lowest lc carryover. Introducing the new acquity uplc iclass plus system. We have 1 waters xevo tqxs mass manual available for free pdf download. The waters acquity uplc hclass bio system is for research use only and is not intended for use in diagnostic applications. Acquity uplc iclass system smftn waters corporation. The acquity uplc i class core system includes a binary solvent manager, a quaternary solvent manager, a sample manager flowthroughneedle, a column heater, detectors fluorescence, photodiode array and fraction collector.
Waters offers a range of training courses that increases your staffs laboratory skills, assists you in integrating new technologies, and ensures that you maximize your investment in missioncritical waters hplc, uplc, and mass spectrometry systems. Intended use of the acquity uplc iclass ivd system the acquity uplc iclass ivd system is a general purpose in vitro diagnostic instrument used to quantify and qualify of a wide variety of. Waters asr catalog waters hilic qc reference material. Uplc hclass system instrument specifications conquer. How to defrost the sample compartment for acquity uplc, hclass and iclass sample manager wkb2920 what is the part number for the inline filter assembly used in an hplc system. Acquity uplc mclass system from waters selectscience. Acquity uplc binary solvent manager available part numbers. Ultraefficient, rapid separations to maximize productivity. Acquity uplc iclass system guide waters corporation. The waters acquity uplc iclass system provides the most powerful solution to the most complex separations and detection challenges. For scientists challenged by the most difficult separations, the. It is intended to be used as a system benchmarking standard.
Calibrate the needles z axis see the acquity uplc online help. See how the acquity uplc hclass flowthroughneedle sample manager enables familiar hplc operation and uplc performance. Waters uplc acquity instrument core system includes. Intended use of the acquity uplc i class ivd system the acquity uplc i class ivd system is a general purpose in vitro diagnostic instrument used to quantify and qualify of a wide variety of. Characterize the needle seal see the acquity uplc online. Class b products are suitable for use in both commercial and. Waters new acquity uplc plus series sets new performance.
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